Enzymatic Enantioselective C–C-Bond Formation in Microreactors

Biotechnology and bioengineering, 2008, 99 (4), 1028-1033

K. Koch, R.J.F. van den berg, P.J. Nieuwland, R. Wijtmans, H. E. Schoemaker, J.C.M. van Hest, F.P.J.T. Rutjes. Radboud University Nijmegen, Institute for Molecules and Materials, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands. 2DSM Pharmaceutical Products—Advanced Synthesis, Catalysis & Development, PO Box 18, NL-6160 MD Geleen, The Netherlands

We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor
setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed.

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