Serpentine channel

SKU
serpentine_config
Availability:
check_circle In stock
As low as $417.33

per pack of 3

Flow cell with a serpentine channel. Details depend on selected configuration.

Application possibilities

This product is designed for observation purposes and offers sublime optical performance in combination with microscopy. This allows high resolution imaging. A regular flow cell offers a limited observation length, using a serpentine shaped channel substantial channel length is created. This allow much longer observation time.  

Research examples can be for instance:

  • Observation of cell interaction with media, like for instance exposure to toxins or active substances.
  • Research on interaction between liquids
  • Observation of a reaction
  • Use as collection reservoir, for instance combined with a PCR reactions

Unique selling point

  • The channels are extremly smooth and are fully optical transparent.
  • Glass offers sublime optical transparency and low autofluorescence.
  • When fluorescent markers are used we have also a D263-Bio version available for even lower autofluorescence in some critical wave length areas.
  • Thin bottom version are compatible with a #1 (11003694  or #1.5 (11003040) coverslip
  • Different size available for a optimal match with cell size used or optimal liquid volume.

Key features of the products 

SKU Number of inlets Number of outlets Channel width Max. cell diameter for optimal observation Channel height Glass height on top of channel Glass height below channel Chip height Glass
11003694 2 1 50µm 10µm 20µm 1100µm 145µm 1245µm D263
11003040 3 1 140µm 40µm 45µm 1100µm  175µm 1275µm D263Bio
11003033 3 1 250µm 110µm 140µm 830µm  830µm 1800µm D263

 

More Information
Unit of measurementpack of 3
Interface typeTopconnect
Chip materialBorosilicate glass
Icon Label Description Type Size Download
pdf Drawing 11003040 Serpentine 45x140µm, 350mm, TB pdf 76.9 KB Download
pdf Drawing 11003033 Serpentine 140x250µm, 500mm pdf 99.7 KB Download
pdf Drawing R50.332.3 Serpentine 20x50µm, 342mm pdf 79.1 KB Download
Customer Questions
How do I clean my uncoated chips?
1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water. 2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages. 3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. 4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements. What can be customized? The channel shape. The channel height and width.For wet etching its important to consider that channel height and minimal channel width are related.<br>{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm}Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels. Surface flatness. We can provide ultra flat surfaces.
I have a Elveflow OB1 pressure based pump what connectors do I need?
You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.
I have a Fluigent pressure based pump, what connectors do I need?
See "Read More" for advice.
When should I use the metal strips (spacers) as provided with the Fluidic Connect Pro holder?
The metal strips (Spacers) are used for thin bottom chips and are also part of the holder for resealable flow cell's. See "Read More" for details.
What's important in selection of a suitable microscope objective?
The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.
What is the roughness of the etched structures?
Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent.
How should I connect the chips to a pump?
Micronit offers a range of chip holders designed to ensure reliable and convenient interfacing between your chip and fluidic tubing. See "Read More"for additional information and links.
Are there alternatives to a chip holder?
While alternatives to a chip holder are available, their implementation is complex and not generally recommended. Further information can be found under “Read More”.
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