Thin bottom flow cell - topconnect

SKU
Flow_cell_conf
Availability:
check_circle In stock
As low as €236.00

per pack of 3

Pack of 3 thin bottom flow cell chips.

Application possibilities

A flow cell / observation cell is a sample cell designed so that liquids sample, for instance containing cell’s, proteins or particles, can be continuously flowed though a observation area.
Our glass flow’s cell are intended for situation where the optical properties are extremely critical and are suitable for high resolution imaging. They are often used in combination with fluorescent markers.

As alternative to continuously flowed sample it’s also possible to apply stopped flow observation by adding pressure control on both the cell inlet and outlet.   

Research examples can be for instance:

  • In-line sampling of liquids, mostly using a optical read out.
  • Cell observation / counting
  • Measurement of fluorescent response.  
  • Combined with surface chemistry they are used as base for NGS technology (Next-Generation Sequencing)

Unique selling point

  • The channels doesn’t have any substantial roughness and are fully optical transparent due to the etching process used.
  • Glass offers sublime optical transparency and low autofluorescence.
  • When fluorescent markers are used we have also a D263-Bio version available for even lower autofluorescence in some critical wave length areas.
  • Compatible with a #1 (11003196, 11003164) or #1.5 (11003133) coverslip
  • Available in different channel dimensions.
  • Available with hydrophobic coating.

Key features of the products 

Item Coating Channel width Number of channels Channel height Thin bottom thickness Material
 
11003133
No coating, hyrophilic surface 500µm, 1500µm, 1000µm 50µm 175µm D263 Bio
 
11006327
Hyrophobic coated 500µm, 1500µm, 1000µm 3 50µm 175µm D263 Bio
11003196 No coating, hyrophilic surface 500µm 3
(same channel size)
50µm 145µm D263 Eco
11006345 Hyrophobic coated 500µm 3
(same channel size)
50µm 145µm D263 Eco
11003164 No coating, hyrophilic surface 1500µm 3
(same channel size)
50µm 145µm D263 Eco
11006338 Hyrophobic coated 1500µm 3
(same channel size)
50µm 145µm D263 Eco

D263 Bio offers a reduced autofluorescence when used in combination with fluorescent markers with emissions above 600nm.

Interfacing
This product is compatible with our Fluidic Connect Pro holder for chips supplied in a cartridge.
Because of the limited chip thickness it’s necessary to use the metal strips included with the holder, see here for more information.
To connect all three channels at the same time, you need six ferrules. The ferrules are available in sets of five. Make sure you order two sets if you want to connect three channels at the same time. 

 

More Information
Unit of measurementpack of 3
Interface typeTopconnect
Channel locationChannels are located 1050µm from the top surface. The distance form the bottom surface is 145µm (version with 3 different channels) and 175µm for the version with the same channels.
Chip thickness1.8mm
Chip materialBorosilicate glass
Number of inlets3
Number of outlets3
Icon Label Description Type Size Download
pdf 11003164 / 11006338 Drawing Flow cell chip 1.5mm width Drawing for Flow cell chip 1.5mm width, items 11003164 (uncoated) / 11006338 (coated) pdf 260.1 KB Download
PDF 11003196 / 11006345 Drawing Flow cell chip 0.5mm width Drawing for Flow cell chip 0.5mm width, items 11003196 (uncoated) / 11006345 (coated) PDF 232.7 KB Download
pdf 11003133 / 11006327 Drawing Flow cell chip variable width 175um bottom Drawing for Flow cell chip variable width 175um bottom, items 11003133 (uncoated) / 11006327 (coated). pdf 206 KB Download
Customer Questions
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements.  What can be customized?

The channel shape. The channel height and width.
For wet etching its important to consider that channel height and minimal channel width are related.
{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm} 
Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels.  Surface flatness. We can provide ultra flat surfaces.
When should I use the metal strips (spacers) as provided with the Fluidic Connect Pro holder?
When should I use the spacers?
The metal strips are used for thin bottom chips and are also part of the holder for resealable flow cell's.
This are currently the following products (list might be incomplete): SKU SKU old Description Note 11000746 00746 Fluidic Connect Pro for resealable flow cells Normally already pre-assembled  11000746 00745 Insert set for resealable flow cells   11003133 03133 Thin bottom flow cell    11106327 03133-F Thin bottom flow cell - coated   11003196 03196 Thin bottom flow cell - 0.5mm   11006345 03196-F Thin bottom flow cell - 0.5mm - coated   11003164 03164 Thin bottom flow cell - 1.5mm   11006338 03164-F Thin bottom flow cell - 1.5mm - coated   11003694 FC_R50.332.3_PACK Serpentine channel / Microreactor, 20x50µm, 342mm length   11003644 FC_R50.332.3-F Serpentine channel / Microreactor, 20x50µm, 342mm length - Coated   11003040 03040 Serpentine channel / Microreactor, 45x140µm, 350mm length     11006834 03040 - F Serpentine channel / Microreactor, 45x140µm, 350mm length - coated   11006722 FC_X3550CH.3_PACK Cross channel chip - thin bottom   How to apply the spacers? The spacers are placed beneath the top insert, as shown in the picture below. What should I do when I don't have spacers available?
Just contact us to request new spacers.
What is the roughness of the etched structures?
Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent. 
Large roughness for structures in glass chips is typical observed for structures manufactured by use of laser assisted manufacturing techniques or abrasion-based techniques like powder blasting. Almost all catalogue products from Micronit are manufactured using wet etching to create full transparent channels without substantial roughness. 
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