H-shaped channel for boundary flow experiment

SKU
config_H
Availability:
check_circle In stock
As low as $409.76

per pack of 3

H-shaped flow channel, ideal for boundary flow experiments.  Available into two diferent channel sizes and optional thin bottom layer.

This H-shaped flow channel is an ideal solution for boundary flow experiments in which two liquids will flow in parallel and only diffusion will occur at the interface of both flows. 

The channels are etched into borosolicate glass which creates extremly smooth channels which are fully transparend.

SKU Channel Width [µm] Channel Height [µm] Bottom layer thickness [µm] Borosilicate glass
11003577 350 150 700 D263-Eco
11003578 120 50 175 D263-Bio

 

More Information
Unit of measurementpack of 3
Interface typeTopconnect
Chip materialBorosilicate glass
Number of inlets2
Number of outlets2
Channel width350µm or 120µm, depending on selection.
CoatingNo coating (hydrophilic)
Customer Questions
How do I clean my uncoated chips?
1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water. 2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages. 3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. 4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.
I need a pumping system for my setup. Which one do you recommend?
We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles.To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers. https://www.fluigent.com/
Which flowrates should I use?
Advice on how to find the right flow rates and to to change the droplet size can be found under "Read More".
I only see streaks of fluids but no droplets. How do I get the droplets?
When you only see streaks of fluids its important to decrease your flowrate. Instructions to find the right flow rate can be found under "Read More".
Which surfactants should I use?
Tween 20, Tween 80 and Emulseo FluoSurf™-O surfactant are used in combination with our chips. See "Read More" for details.
How do I prevent clogging of my chips?
Clogging can only be avoided by working as cleanly as possible. Typical sources of particles include: - Dust from the air or open surfaces - Larger particles or other contamination in the fluids - Dried or solidified residues when re-using chips from previous runs - Components within the fluidic setup (e.g., tubing, connectors, reservoirs) - Residual particles remaining after cleaning See “Read More” for detailed advice on minimizing these risks.
Should I use coated or uncoated droplet generators?
For water-in-oil droplets, use droplet generators with a hydrophobic coating.For oil-in-water droplets, no additional coating is needed. Below more details on surface wetting properties are explained.Surface wetting propertiesThe contact angle between the fluid and the droplet generator surface is key for defining the stability of the wetting of the continuous phase. If the wetting is more preferential for the dispersed phase, pinning of the droplets to the channel can occur. The standard uncoated droplet generators of Micronit are glass based, thus hydrophilic and are suitable for making organic droplets in an aqueous phase (oil-in-water droplets). It is possible for Micronit to provide a coating which renders the surface of the droplet generators hydrophobic, thus suitable for making aqueous droplets in an organic phase (water-in-oil droplets). This coating is based on a fluorinated polymer and ensures the coated surface has a contact angle of more than 90° with water.
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements.  What can be customized? The channel shape. The channel height and width. For wet etching its important to consider that channel height and minimal channel width are related.{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm} Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels.  Surface flatness. We can provide ultra flat surfaces.
I have a Elveflow OB1 pressure based pump what connectors do I need?
You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.
I have a Fluigent pressure based pump, what connectors do I need?
See "Read More" for advice.
What's important in selection of a suitable microscope objective?
The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.
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