H-shaped channel for boundary flow experiment

1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water. 2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages. 3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. 4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.

Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements. What can be customized? The channel shape. The channel height and width.For wet etching its important to consider that channel height and minimal channel width are related.<br>{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm}Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels. Surface flatness. We can provide ultra flat surfaces.

You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.

See "Read More" for advice.

The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.

Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent.

Micronit offers a range of chip holders designed to ensure reliable and convenient interfacing between your chip and fluidic tubing. See "Read More"for additional information and links.

While alternatives to a chip holder are available, their implementation is complex and not generally recommended. Further information can be found under “Read More”.