Thin bottom flow cell, 75x25mm

SKU
FLC7525
Availability:
check_circle In stock
As low as €645.00

per pack of 2

Pack of 2, microscope slide format (75x25mm) thin botom flow cells

+ 0

Application possibilities

A flow cell / observation cell is a sample cell designed so that liquids sample, for instance containing cell’s, proteins or particles, can be continuously flowed though a observation area.
Our glass flow’s cell are intended for situation where the optical properties are extremely critical and are suitable for high resolution imaging. They are often used in combination with fluorescent markers.

As alternative to continuously flowed sample it’s also possible to apply stopped flow observation by adding pressure control on both the cell inlet and outlet.   

Research examples can be for instance:

  • In-line sampling of liquids, mostly using a optical read out.
  • Cell observation / counting
  • Measurement of fluorescent response.  
  • Combined with surface chemistry they are used as base for NGS technology (Next-Generation Sequencing)

Unique selling point

  • The channels doesn’t have any substantial roughness and are fully optical transparent due to the etching process used.
  • Glass offers sublime optical transparency and low autofluorescence.
  • D263-Bio is the material of choice for when fluorescent markers are used, this material featurea a very low autofluorescence also above 600nm.
  • The bottom layer thickness (thickness from bottom face up to the channel) is comparable with a #1.5 coverslip
  • Optionally available with a hydrophobic or APTES coating.
  • The miscroscope slide format (75x25mm) offers compared to a 45x15mm format:
    • more channels per chips (5 instead of 3)
    • longer observation length (+30mm)

Product configurations

SKU Channel 1 width Channel 2 width Channel 3 width Channel 4 width Channel 5 width Channel height (all channels) Coating
11003545 1400µm 1900µm 2900µm 1900µm 1400µm 50µm Non, hydrophobic
11003536 1500µm 2000µm 3000µm 2000µm 1500µm 100µm Non, hydrophobic

Interfacing
This product requires an alternative Catalog Product Link for use in combination with our Fluidic connect Pro holder.
To connect all three channels at the same time, you need 10 ferrules. The ferrules are available in sets of five. Make sure you order two sets if you want to connect all channels at the same time. 

More Information
Unit of measurementpack of 2
Interface typeTopconnect
Channel location175µm from bottom of chips
Chip thickness1.3mm
Chip materialBorosilicate glass - D263 bio
Number of inlets5
Number of outlets5
Icon Label Description Type Size Download
pdf Drawing FC 7525.100.3 Technical drawing for 75x25mm flow cell, 100µm deep. pdf 85.2 KB Download
PDF Drawing FC 7525.50.3 Technical drawing for 75x25mm flow cell, 50µm deep. PDF 133.7 KB Download
Customer Questions
How do I clean my chips?
One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well, but a lower concentration might also be sufficient. If traces of the cleaning solution remain inside the chip after cleaning, rinse with water or ammonia. Further, plastic parts should not be exposed to alkaline solutions.
To remove particulate matter from your chip, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels.
Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it.
Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions. Always keep in mind that you are working with extremely corrosive material. Please note that this instruction is focused on the chip itself, PEEK elements like connectors are not so compatible with strong sulforic acid.
Please note that chips that were coated by Micronit have different guidelines for cleaning!
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements.  What can be customized?

The channel shape. The channel height and width.
For wet etching its important to consider that channel height and minimal channel width are related.
{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm} 
Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels.  Surface flatness. We can provide ultra flat surfaces.
What is the roughness of the etched structures?
Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent. 
Large roughness for structures in glass chips is typical observed for structures manufactured by use of laser assisted manufacturing techniques or abrasion-based techniques like powder blasting. Almost all catalogue products from Micronit are manufactured using wet etching to create full transparent channels without substantial roughness. 
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