Organ-on-a-chip membrane layer - 3µm

SKU
11001206
Availability:
Produced after order, 3 weeks lead time.
$1,231.57

per pack of 12

Pack of 12 middle layers, to be placed in between top and bottom organ-on-a-chip layers.

Note: this is a make to order item. Our aim is to ship within 3-4 weeks after receiving the order.

In organ-on-a-chip (OOC) processes, a cell culture membrane is placed in between two resealable glass slides to form two separate flow chambers. This allows two different flows, of either liquids or gases, on either side of the membrane. This dynamic microfluidic flow approach enables new and innovative ways to culture cells and tissues while offering precise and continuous control during the complete culturing process. The membrane layer together with a top and bottom OOC layer makes an organ-on-a-chip resealable flow cell.

The details of this layer are:

Pore size
[µm]

Membrane thickness
[µm]
Pore density
[per cm2]
3 9 8.00E+5

 

The pores are created using track-etching and have a random orientation:

Track etched pores (top view)

 

Using a small angle the random direction is shown:

More Information
Unit of measurementpack of 12
Interface typeTopconnect
Membrane poresize3µm
Carrier thickness0.4mm
Carrier materialBorosilicate glass
Membrane materialPET
Membrane appearanceTransparent
Membrane surface treatmentCell culture treated
Icon Label Description Type Size Download
pdf Organ-on-a-chip drawing pdf 118.4 KB Download
pdf Organ-on-a-chip manual Manual that helps to get started with OOC using the resealable platform. pdf 1.5 MB Download
pdf Compatibility with cell imaging systems pdf 153.1 KB Download
Customer Questions
Is it possible to monitor oxygen levels?
It is possible to couple Micronit OOC devices to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of oxygen in the culture chamber.
Is my OOC system compatible with my imaging systems?
A document link can be found under "Read More".
The membrane is moving and flow is switching between top and bottom flow path. What is happening?
Always check that there’s no blockage in one of the flow paths, this can be verified by checking the amount of collected liquid in a specific time frame and compare it with the expected amount for the flow rate used.If this isn't the case, droplet formation on the end of the tubing is the problem. Each falling droplet affects the flow a bit, resulting in changes in flow rates. Resolve this by keeping the end of the tubing in the collection reservoir submerged in liquid.
How do I clean my resealable flow cell's / OOC top and bottom layers?
The glass layer without a gasket can be cleaned using the same methods commonly used for glass microscope slides. The gasket layer, however, requires more careful handling. For detailed instructions, see "Read More".
How do I sterilize my resealable flow cell's / OOC top and bottom layers?
Advice on sterilization of glass slides and glass slides without gasket can be found under "Read More".
Publication: Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners.
Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners. Kulthong K, Duivenvoorde L, Mizera BZ, Rijkers D, Dam GT, Oegema G, Puzyn T, Bouwmeester H, Van Der Zande M. RSC Advances. 2018; 8(57): 32440-32453 https://doi.org/10.1039/C8RA05430D
Publication: Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models.
Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models. Kulthong K, Duivenvoorde L, Sun H, Confederat S, Wu J, Spenkelink B, de Haan L, Marin V, van der Zande M, Bouwmeester H. Toxicol In Vitro. 2020 Jun;65:104815 https://doi.org/10.1016/j.tiv.2020.104815
Publication: Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia.
Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia. Kulthong K, Hooiveld GJEJ, Duivenvoorde L, Miro Estruch I, Marin V, van der Zande M, Bouwmeester H. Sci Rep. 2021 Feb 5;11(1):3234 https://doi: 10.1038/s41598-021-82853-6
Publication: Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions.
Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions Duivenvoorde LPM, Louisse J, Pinckaers NET, Nguyen T, van der Zande M. Sci Rep. 2021 May 14;11(1):10327. https://www.nature.com/articles/s41598-021-89710-6
What pump system should I use for a resealable flow cell or OOC chips?
It's important that the pressure system can limit the amount of pressure that will be applied. We would recommend a pressure based system. However it should be possible to use a syringe pump when a pressure relief valve is applied for each liquid path.
Would you like to submit a question? Please login first.
Copyright © 2020-present Micronit B.V. All rights reserved.