Organ-on-a-chip top and bottom layers

Name: Organ-on-a-chip top and bottom layers
SKU: 11000739
Price: 893.94 USD

SKU

11000739

Availability:

check_circle In stock

$893.94

per pack of 4

Pack with resealable glass slides that form the top and bottom layers for organ-on-a-chip devices. This pack contains 4 top and 4 bottom layers.

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Details

Two resealable glass slides that form the top and bottom layers for organ-on-a-chip devices. When the membrane layer is placed in between these top and bottom layers, two separate flow chambers are formed. With this item, use the Fluidic Connect Pro holder for resealable flow cells.

The organ-on-a-chip top and bottom layers are intended for low pressure aplications, up to 300-400mbar. It's strongly recommend to use a pump system with addequate pressure control to assure the pressure will not exceed this value or use a pressure relieve valve.

More Information

More Information
Unit of measurement	pack of 4
Interface type	Topconnect
Carrier thickness	0.4mm
Carrier material	Borosilicate glass
Membrane material	PET
Membrane appearance	Transparent
Membrane surface treatment	Cell culture treated

Documents

Label	Description	Type	Size	Download
Organ-on-a-chip manual	Manual that helps to get started with OOC using the resealable platform.	pdf	1.5 MB	Download
Organ-on-a-chip drawing		pdf	118.4 KB	Download
Compatibility with cell imaging systems		pdf	153.1 KB	Download

Product Questions (14)

Customer Questions

I have a Elveflow OB1 pressure based pump what connectors do I need?

You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.

I have a Fluigent pressure based pump, what connectors do I need?

See "Read More" for advice.

What's important in selection of a suitable microscope objective?

The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.

What pump system should I use for a resealable flow cell or OOC chips?

It's important that the pressure system can limit the amount of pressure that will be applied. We would recommend a pressure based system. However it should be possible to use a syringe pump when a pressure relief valve is applied for each liquid path.

How do I sterilize my resealable flow cell's / OOC top and bottom layers?

Advice on sterilization of glass slides and glass slides without gasket can be found under "Read More".

How do I clean my resealable flow cell's / OOC top and bottom layers?

The glass layer without a gasket can be cleaned using the same methods commonly used for glass microscope slides. The gasket layer, however, requires more careful handling. For detailed instructions, see "Read More".

Is it possible to monitor oxygen levels?

It is possible to couple Micronit OOC devices to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of oxygen in the culture chamber.

Is my OOC system compatible with my imaging systems?

A document link can be found under "Read More".

What is the oxygen permeability data for the elastomer used?

More details on oxygen permeability can be found under "Read More".

The membrane is moving and flow is switching between top and bottom flow path. What is happening?

Always check that there’s no blockage in one of the flow paths, this can be verified by checking the amount of collected liquid in a specific time frame and compare it with the expected amount for the flow rate used.If this isn't the case, droplet formation on the end of the tubing is the problem. Each falling droplet affects the flow a bit, resulting in changes in flow rates. Resolve this by keeping the end of the tubing in the collection reservoir submerged in liquid.

Publication: Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners.

Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners. Kulthong K, Duivenvoorde L, Mizera BZ, Rijkers D, Dam GT, Oegema G, Puzyn T, Bouwmeester H, Van Der Zande M. RSC Advances. 2018; 8(57): 32440-32453 https://doi.org/10.1039/C8RA05430D

Publication: Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models.

Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models. Kulthong K, Duivenvoorde L, Sun H, Confederat S, Wu J, Spenkelink B, de Haan L, Marin V, van der Zande M, Bouwmeester H. Toxicol In Vitro. 2020 Jun;65:104815 https://doi.org/10.1016/j.tiv.2020.104815

Publication: Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia.

Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia. Kulthong K, Hooiveld GJEJ, Duivenvoorde L, Miro Estruch I, Marin V, van der Zande M, Bouwmeester H. Sci Rep. 2021 Feb 5;11(1):3234 https://doi: 10.1038/s41598-021-82853-6

Publication: Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions.

Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions Duivenvoorde LPM, Louisse J, Pinckaers NET, Nguyen T, van der Zande M. Sci Rep. 2021 May 14;11(1):10327. https://www.nature.com/articles/s41598-021-89710-6

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