OOC setup (no pumps included)

It is possible to couple Micronit OOC devices to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of oxygen in the culture chamber.

A document link can be found under "Read More".

Always check that there’s no blockage in one of the flow paths, this can be verified by checking the amount of collected liquid in a specific time frame and compare it with the expected amount for the flow rate used.If this isn't the case, droplet formation on the end of the tubing is the problem. Each falling droplet affects the flow a bit, resulting in changes in flow rates. Resolve this by keeping the end of the tubing in the collection reservoir submerged in liquid.

The glass layer without a gasket can be cleaned using the same methods commonly used for glass microscope slides. The gasket layer, however, requires more careful handling. For detailed instructions, see "Read More".

Advice on sterilization of glass slides and glass slides without gasket can be found under "Read More".

More details on oxygen permeability can be found under "Read More".

You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.

See "Read More" for advice.

Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners. Kulthong K, Duivenvoorde L, Mizera BZ, Rijkers D, Dam GT, Oegema G, Puzyn T, Bouwmeester H, Van Der Zande M. RSC Advances. 2018; 8(57): 32440-32453 https://doi.org/10.1039/C8RA05430D

Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models. Kulthong K, Duivenvoorde L, Sun H, Confederat S, Wu J, Spenkelink B, de Haan L, Marin V, van der Zande M, Bouwmeester H. Toxicol In Vitro. 2020 Jun;65:104815 https://doi.org/10.1016/j.tiv.2020.104815

Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia. Kulthong K, Hooiveld GJEJ, Duivenvoorde L, Miro Estruch I, Marin V, van der Zande M, Bouwmeester H. Sci Rep. 2021 Feb 5;11(1):3234 https://doi: 10.1038/s41598-021-82853-6

Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions Duivenvoorde LPM, Louisse J, Pinckaers NET, Nguyen T, van der Zande M. Sci Rep. 2021 May 14;11(1):10327. https://www.nature.com/articles/s41598-021-89710-6

The available view window is 25x12mm, see "Read More" for details.