Hydrophobic Serpentine channel / Microreactor, 20x50µm, 342mm length

SKU
11003644
Availability:
Coated on request 3-4 weeks lead time.
$374.52

per pack of 3

Hydrophobic coated flow cell with a serpentine channel with a length of 342mm. Channel width: 50µm, channel height: 20µm.
The chip has a 145µm thick bottom and is manufactured from D263 glass.

Application possibilities
This product is designed for observation purposes and offers sublime optical performance in combination with microscopy. This allows high resolution imaging.

Unique selling points

  • Hydrophobic channel surface, due to coating.
  • For cell up to 10µm in diameter.
  • 342mm of observation length, 26 meanders of 12mm
  • Thin bottom, 145µm channel bottom thickness (#1 coverslip)
  • Low autofluorescence.
  • Manufactured from high-end glass, channels are extremely smooth and fully transparent.

Key features of the product are: 

SKU Number of inlets Number of outlets Channel width Flat channel area width Channel height Glass height on top of channel Glass height below channel Chip height
11003644 2 1 50µm 10µm 20µm 1100µm  145µm 1245µm

Coating
The selected products contains a hydrophobic coating. This coating will change the contact angle between channel surfaces and the liquid.  
This coating can influence the binding of proteins and other biomolecules. There's currently no information available if autofluorescence is influenced.

Interfacing
This product is compatible with our Fluidic Connect Pro holder for chips supplied in a cartridge.
Because of the limited chip thickness it’s necessary to use the metal strips included with the holder, see here for more information.

 

More Information
Unit of measurementpack of 3
Interface typeTopconnect
Chip materialBorosilicate glass
Chip thickness1245µm
Number of inlets2
Number of outlets1
Channel width50µm
Internal volumeInternal volume 0.3µl
Bottom thickness145µm bottom layer
Max. cell height10µm
CoatingHydrophobic coating
Icon Label Description Type Size Download
pdf Drawing R50.332.3 Serpentine 20x50µm, 342mm pdf 79.1 KB Download
Customer Questions
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements.  What can be customized?

The channel shape. The channel height and width.
For wet etching its important to consider that channel height and minimal channel width are related.
{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm} 
Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels.  Surface flatness. We can provide ultra flat surfaces.
When should I use the metal strips (spacers) as provided with the Fluidic Connect Pro holder?
When should I use the spacers?
The metal strips are used for thin bottom chips and are also part of the holder for resealable flow cell's.
This are currently the following products (list might be incomplete): SKU SKU old Description Note 11000746 00746 Fluidic Connect Pro for resealable flow cells Normally already pre-assembled  11000746 00745 Insert set for resealable flow cells   11003133 03133 Thin bottom flow cell    11106327 03133-F Thin bottom flow cell - coated   11003196 03196 Thin bottom flow cell - 0.5mm   11006345 03196-F Thin bottom flow cell - 0.5mm - coated   11003164 03164 Thin bottom flow cell - 1.5mm   11006338 03164-F Thin bottom flow cell - 1.5mm - coated   11003694 FC_R50.332.3_PACK Serpentine channel / Microreactor, 20x50µm, 342mm length   11003644 FC_R50.332.3-F Serpentine channel / Microreactor, 20x50µm, 342mm length - Coated   11003040 03040 Serpentine channel / Microreactor, 45x140µm, 350mm length     11006834 03040 - F Serpentine channel / Microreactor, 45x140µm, 350mm length - coated   11006722 FC_X3550CH.3_PACK Cross channel chip - thin bottom   How to apply the spacers? The spacers are placed beneath the top insert, as shown in the picture below. What should I do when I don't have spacers available?
Just contact us to request new spacers.
What is the roughness of the etched structures?
Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent. 
Large roughness for structures in glass chips is typical observed for structures manufactured by use of laser assisted manufacturing techniques or abrasion-based techniques like powder blasting. Almost all catalogue products from Micronit are manufactured using wet etching to create full transparent channels without substantial roughness. 
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