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    • T-shaped droplet generator - topconnect

    SKU
    t-shaped
    Availability:
    check_circle In stock
    As low as $649.46

    per pack of 3

    Pack of three T-shaped droplet generator chips.

    main product photo

    Application area

    Microfluidic droplet generators are excellent tools for generating highly reproducible microsized droplets with much higher precision and repeatability compared to conventional methods.

    Such droplet can be aplied for:

    • Emulsification of a oil and water like substance 
    • Cell encapsulation, a (single) cell can be encapsulated to create it's own micro-environment and makes it easier to disinguish individual cell's. 
    • Creation of microparticles.

    Chip details

    The T-shaped dropletgnerator is the most classical type of droplet generator. By tuning the relative viscosities, surface tension, and velocities between the dispersed and the continuous phase, droplets of almost any size can be created.

    Oil-in-Water (O/W) droplets can be generated directly using the glass chips. For Water-in-Oil droplets (W/O), choose the coated chips.

    Chip design

    The chip design is as follow:

    More details can be found in the drawing.

    The channels have a height of 20µm and width of 100µm and are etched in a single layer. This gives the following channel profile:

     

    The chips contains a nozzle (half semi-oval) with a size of 34x10µm (width x height) that connects the sub-channel with the main-channel. 

     

    More Information
    More Information
    Unit of measurementpack of 3
    Interface typeTopconnect
    Chip materialBorosilicate glass
    Supply format & dimensions

    Supplied in a black polymer cardtridge with an external dimension of 75 x 25mm.
    The functional glass element has a size of 45x15mm.

    Chip thickness1800µm
    Number of inlets2
    Number of outlets1
    Documents
    Icon Label Description Type Size Download
    pdf TSDG.2 - T-shaped droplet generator pdf 67.3 KB Download
    pdf Surface wetting properties pdf 220.3 KB Download
    pdf Flow rate instructions pdf 303.6 KB Download
    pdf How to prevent clogging pdf 153 KB Download
    pdf Using the right surfactants pdf 168.8 KB Download
    Product Questions (12)
    Customer Questions
    How do I clean my uncoated chips?
    1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water. 2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages. 3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. 4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.
    Read More
    I need a pumping system for my setup. Which one do you recommend?
    We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles.To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers. https://www.fluigent.com/
    Read More
    Which flowrates should I use?
    Advice on how to find the right flow rates and to to change the droplet size can be found under "Read More".
    Read More
    I only see streaks of fluids but no droplets. How do I get the droplets?
    When you only see streaks of fluids its important to decrease your flowrate. Instructions to find the right flow rate can be found under "Read More".
    Read More
    Which surfactants should I use?
    Tween 20, Tween 80 and Emulseo FluoSurf™-O surfactant are used in combination with our chips. See "Read More" for details.
    Read More
    How do I prevent clogging of my chips?
    Clogging can only be avoided by working as cleanly as possible. Typical sources of particles include: - Dust from the air or open surfaces - Larger particles or other contamination in the fluids - Dried or solidified residues when re-using chips from previous runs - Components within the fluidic setup (e.g., tubing, connectors, reservoirs) - Residual particles remaining after cleaning See “Read More” for detailed advice on minimizing these risks.
    Read More
    Should I use coated or uncoated droplet generators?
    For water-in-oil droplets, use droplet generators with a hydrophobic coating.For oil-in-water droplets, no additional coating is needed. Below more details on surface wetting properties are explained.Surface wetting propertiesThe contact angle between the fluid and the droplet generator surface is key for defining the stability of the wetting of the continuous phase. If the wetting is more preferential for the dispersed phase, pinning of the droplets to the channel can occur. The standard uncoated droplet generators of Micronit are glass based, thus hydrophilic and are suitable for making organic droplets in an aqueous phase (oil-in-water droplets). It is possible for Micronit to provide a coating which renders the surface of the droplet generators hydrophobic, thus suitable for making aqueous droplets in an organic phase (water-in-oil droplets). This coating is based on a fluorinated polymer and ensures the coated surface has a contact angle of more than 90° with water.
    Read More
    I have a Elveflow OB1 pressure based pump what connectors do I need?
    You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.
    Read More
    I have a Fluigent pressure based pump, what connectors do I need?
    See "Read More" for advice.
    Read More
    What's important in selection of a suitable microscope objective?
    The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.
    Read More
    Does those droplet generators work well with Emulseo surfactants and Oils?
    Micronit hydrophobic coated Glass Droplet generators are tested with the combination of Emulseo FluoSurf-O™ surfactant and Emulseo FluoOil™ 7500 oil and good results are obtained.
    Read More
    Can I apply a hydrophobic coating myself using Emulseo Fluo-ST3™ surface treatment?
    The combination of Micronit glass droplet generators and Emulseo Fluo-ST3™ surface treatment is tested and works well.
    Read More
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