Droplet generation setup - topconnect (no pumps included)

One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well, but a lower concentration might also be sufficient. If traces of the cleaning solution remain inside the chip after cleaning, rinse with water or ammonia. Further, plastic parts should not be exposed to alkaline solutions.
To remove particulate matter from your chip, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels.
Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it.
Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions. Always keep in mind that you are working with extremely corrosive material. Please note that this instruction is focused on the chip itself, PEEK elements like connectors are not so compatible with strong sulforic acid.
Please note that chips that were coated by Micronit have different guidelines for cleaning!

We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles. To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers: https://www.fluigent.com/  

This depends on many things. For example on the type of fluid that you are using. Check our flowrate instructions to find out how to start.

Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.

Use our surfactant guide for advice on surfactants.

Have a look at our clogging prevention guide.

Have a look at our article about surface wetting properties.

Amoyav, Benzion, and Ofra Benny "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10. Abstract Microfluidics technology offers a new platform to control liquids under flow in small volumes. The advantage of using smallscale reactions for droplet generation along with the capacity to control the preparation parameters, making microfluidic chips an attractive technology for optimizing encapsulation formulations. However, one of the drawbacks in this methodology is the ability to obtain a wide range of droplet sizes, from sub-micron to microns using a single chip design. In fact, typically, droplet chips are used for micron-dimension particles, while nanoparticles’ synthesis requires complex chips design (i.e., microreactors and staggered herringbone micromixer). Here, we introduce the development of a highly tunable and controlled encapsulation technique, using two polymer compositions, for generating particles ranging from microns to nano-size using the same simple single microfluidic chip design. Poly(lactic-co-glycolic acid) (PLGA 50:50) or PLGA/polyethylene glycol polymeric particles were prepared with focused-flow chip, yielding monodisperse particle batches. We show that by varying flow rate, solvent, surfactant and polymer composition, we were able to optimize particles’ size and decrease polydispersity index, using simple chip designs with no further related adjustments or costs. Utilizing this platform, which offers tight tuning of particle properties, could offer an important tool for formulation development and can potentially pave the way towards a better precision nanomedicine. Keywords: Microfluidics · Nanoparticles · Microparticles · Polymeric particles · Focused flow

Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022. Abstract Multicellular spheroids serve as an excellent platform to study tissue behavior and tumor growth in a controlled, three-dimensional (3D) environment. While molecular and cellular studies have long used this platform to study cell behavior in 3D, only recently have studies using multicellular spheroids shown an important role for the mechanics of the microenvironment in a wide range of cellular processes, including during tumor progression. Despite the well-established relevance of mechanical cues to cell behavior and the numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variations in endogenous cellular forces within growing multicellular aggregates remain unknown. Using cell-sized oil droplets with controlled physicochemical properties as force transducers in mesenchymal cell aggregates, we show that the magnitude of cell-generated stresses varies only weakly with spatial location within the spherical aggregate, but it increases considerably over time during aggregate compaction and growth. Moreover, our results indicate that the temporal increase in cellular stresses is due to increasing cell pulling forces transmitted via integrin-mediated cell adhesion, consistent with the need for larger intercellular pulling forces to compact cell aggregates.

Nuts and ferrule
Elveflow uses 1/4-28 screw in connections on there reservoirs and sensors. To interface you will need Flangeless nuts 1/4-28 for 1/16" tubing, including compatible ferrules. Those nuts and ferrules are included in Elveflows "Kit Fittings Starter Pack Push-in".
This item wil look like this:
In addition you will need our connection kit. Our Connection kit includes nuts or ferrules specificilly designed for our Fluidic Connect Pro, Fluidic Connect 4515 or side connect holders. Tubing included in "Kit Fittings Starter Pack Push-in"
The 1/16"OD PTFE tubing included in the kit has a very large ID (1/32" ~0.79mm) and is from a relatively soft material, this is not so suitable for bot our Fluidic Connect Pro and Fluidic Connect 4515 holders as there's very limited wall thickness. We recommend alternative tubing, like ETFE with a maximum of 0.5mm ID. This tubing is included in our connection kits.
Use of 1/32" OD tubing
1/32" OD tubing can be in combination with our Fluidic Connect Pro holder also be used, but need alternative ferrules.
The ElveFlow "Kit Fittings Starter Pack Push-in" doesn't include sleeves for use of 1/32" OD tubing. They are included in our Syringe to tube connection kit
Our Sideconnect our Fluidic Connect 4515 holders doens't support 1/32" tubing.

Lineup We recommend the dafault Fluigent kits (FLOW EZ™ SUPPLY KIT, FLUIWELL KIT or P-CAP KIT)
The P-cab or FLUIWELL KIT already contains 0.5m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order: For Fluidic Connect Pro our propriatary ferrules.  For Fluidic Connect 4515 our propriatary flatbottom nuts and ferrules.  For Sideconnect our propriatary ferrules.  For optimal performance we recommend our complete connection kit, the FEP tubing might be a bit soft limiting the pressure rating.  MFCS We recommend the dafault Fluigent kits (MFCS™-EZ HIGH/LOW PRESSURE KIT, FLUIWELL KIT or P-CAP KIT )  The P-cab or FLUIWELL KIT already contains 0.5m or 1m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order: For Fluidic Connect Pro our propriatary ferrules.  For Fluidic Connect 4515 our propriatary flatbottom nuts and ferrules.  For Sideconnect our propriatary ferrules.  For optimal performance we recommend our complete connection kit, the FEP tubing might be a bit soft limiting the pressure rating.