Classic focused flow droplet generator

Name: Classic focused flow droplet generator
SKU: FFDROP_CLASIC
Price: 649.46 USD

SKU

FFDROP_CLASIC

Availability:

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As low as $649.46

per pack of 3

Classic droplet generator. The channels have half semi-oval shape.

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Details

Droplet generation is a important technique for emulsification and single cell encapsultation. Also in Pharmaceutical applications, droplet generation can play an important role in drug delivery, when active ingredients are mixed with biological polymers.

Micornit offers currently two versions of single junction focused flow droplet generators, we offer a "classic" and a new generation version. In most case the new generation droplet generators are the prefered choice. However the classic version helps to determine if a half semi-oval channel shape can be sufficient as this is relatively cost effective design. Because it's was one of the first commercial availble droplet generatore chips it's also referenced in a large amount of publications.

Half semi-oval channel shape ("classic") Full semi-oval channel shape ("new generation")

The "classic" droplet generator is available in the following configurations:

SKU	Nozzle height [µm]	Hydrophobic Coating	Channel height [µm]	Channel width [µm]
02889	10	No	20	100
02892	10	Yes	20	100
02890	50	No	100	500
02891	50	Yes	100	500

More details are displayed when the desired configuration is selected.

More Information

More Information
Unit of measurement	pack of 3
Interface type	Topconnect - product specific
Chip material	Borosilicate glass

Product Questions (15)

Customer Questions

How do I clean my uncoated chips?

1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water. 2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages. 3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. 4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.

I need a pumping system for my setup. Which one do you recommend?

We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles.To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers. https://www.fluigent.com/

Which flowrates should I use?

Advice on how to find the right flow rates and to to change the droplet size can be found under "Read More".

I only see streaks of fluids but no droplets. How do I get the droplets?

When you only see streaks of fluids its important to decrease your flowrate. Instructions to find the right flow rate can be found under "Read More".

Which surfactants should I use?

Tween 20, Tween 80 and Emulseo FluoSurf™-O surfactant are used in combination with our chips. See "Read More" for details.

How do I prevent clogging of my chips?

Clogging can only be avoided by working as cleanly as possible. Typical sources of particles include: - Dust from the air or open surfaces - Larger particles or other contamination in the fluids - Dried or solidified residues when re-using chips from previous runs - Components within the fluidic setup (e.g., tubing, connectors, reservoirs) - Residual particles remaining after cleaning See “Read More” for detailed advice on minimizing these risks.

Should I use coated or uncoated droplet generators?

For water-in-oil droplets, use droplet generators with a hydrophobic coating.For oil-in-water droplets, no additional coating is needed. Below more details on surface wetting properties are explained.Surface wetting propertiesThe contact angle between the fluid and the droplet generator surface is key for defining the stability of the wetting of the continuous phase. If the wetting is more preferential for the dispersed phase, pinning of the droplets to the channel can occur. The standard uncoated droplet generators of Micronit are glass based, thus hydrophilic and are suitable for making organic droplets in an aqueous phase (oil-in-water droplets). It is possible for Micronit to provide a coating which renders the surface of the droplet generators hydrophobic, thus suitable for making aqueous droplets in an organic phase (water-in-oil droplets). This coating is based on a fluorinated polymer and ensures the coated surface has a contact angle of more than 90° with water.

Publication: Controlled and tunable polymer particles' production using a single microfluidic device

Amoyav, Benzion, and Ofra Benny. "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10.

Publication: Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids

Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022.

I have a Elveflow OB1 pressure based pump what connectors do I need?

You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.

I have a Fluigent pressure based pump, what connectors do I need?

See "Read More" for advice.

What's important in selection of a suitable microscope objective?

The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.

What is the roughness of the etched structures?

Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent.

Does those droplet generators work well with Emulseo surfactants and Oils?

Micronit hydrophobic coated Glass Droplet generators are tested with the combination of Emulseo FluoSurf-O™ surfactant and Emulseo FluoOil™ 7500 oil and good results are obtained.

Can I apply a hydrophobic coating myself using Emulseo Fluo-ST3™ surface treatment?

The combination of Micronit glass droplet generators and Emulseo Fluo-ST3™ surface treatment is tested and works well.

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Classic focused flow droplet generator

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