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Product group: OOC
I have a Elveflow OB1 pressure based pump what connectors do I need?
You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.
I have a Fluigent pressure based pump, what connectors do I need?
See "Read More" for advice.
What's important in selection of a suitable microscope objective?
The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.
What pump system should I use for a resealable flow cell or OOC chips?
It's important that the pressure system can limit the amount of pressure that will be applied. We would recommend a pressure based system.
However it should be possible to use a syringe pump when a pressure relief valve is applied for each liquid path.
How should I connect the chips to a pump?
Micronit offers a range of chip holders designed to ensure reliable and convenient interfacing between your chip and fluidic tubing. See "Read More"for additional information and links.
How do I sterilize my resealable flow cell's / OOC top and bottom layers?
Advice on sterilization of glass slides and glass slides without gasket can be found under "Read More".
How do I clean my resealable flow cell's / OOC top and bottom layers?
The glass layer without a gasket can be cleaned using the same methods commonly used for glass microscope slides. The gasket layer, however, requires more careful handling. For detailed instructions, see "Read More".
What are the dimension of the available view window using the resealable / OOC chip holder?
The available view window is 25x12mm, see "Read More" for details.
Is it possible to monitor oxygen levels?
It is possible to couple Micronit OOC devices to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of oxygen in the culture chamber.
Is my OOC system compatible with my imaging systems?
A document link can be found under "Read More".
What is the oxygen permeability data for the elastomer used?
More details on oxygen permeability can be found under "Read More".
The membrane is moving and flow is switching between top and bottom flow path. What is happening?
Always check that there’s no blockage in one of the flow paths, this can be verified by checking the amount of collected liquid in a specific time frame and compare it with the expected amount for the flow rate used.If this isn't the case, droplet formation on the end of the tubing is the problem. Each falling droplet affects the flow a bit, resulting in changes in flow rates. Resolve this by keeping the end of the tubing in the collection reservoir submerged in liquid.
Publication: Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners.
Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners.
Kulthong K, Duivenvoorde L, Mizera BZ, Rijkers D, Dam GT, Oegema G, Puzyn T, Bouwmeester H, Van Der Zande M.
RSC Advances. 2018; 8(57): 32440-32453
https://doi.org/10.1039/C8RA05430D
Publication: Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models.
Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models.
Kulthong K, Duivenvoorde L, Sun H, Confederat S, Wu J, Spenkelink B, de Haan L, Marin V, van der Zande M, Bouwmeester H.
Toxicol In Vitro. 2020 Jun;65:104815
https://doi.org/10.1016/j.tiv.2020.104815
Publication: Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia.
Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia.
Kulthong K, Hooiveld GJEJ, Duivenvoorde L, Miro Estruch I, Marin V, van der Zande M, Bouwmeester H.
Sci Rep. 2021 Feb 5;11(1):3234
https://doi: 10.1038/s41598-021-82853-6
Publication: Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions.
Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions
Duivenvoorde LPM, Louisse J, Pinckaers NET, Nguyen T, van der Zande M.
Sci Rep. 2021 May 14;11(1):10327.
https://www.nature.com/articles/s41598-021-89710-6
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