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Product Group: Flow cells
How do I clean my uncoated chips?
1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water.
2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages.
3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick.
4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements.
What can be customized?
The channel shape.
The channel height and width.For wet etching its important to consider that channel height and minimal channel width are related.<br>{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm}Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common.
Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels.
Surface flatness. We can provide ultra flat surfaces.
I have a Elveflow OB1 pressure based pump what connectors do I need?
You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.
I have a Fluigent pressure based pump, what connectors do I need?
See "Read More" for advice.
When should I use the metal strips (spacers) as provided with the Fluidic Connect Pro holder?
The metal strips (Spacers) are used for thin bottom chips and are also part of the holder for resealable flow cell's.
See "Read More" for details.
What's important in selection of a suitable microscope objective?
The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.
What is the roughness of the etched structures?
Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent.
How should I connect the chips to a pump?
Micronit offers a range of chip holders designed to ensure reliable and convenient interfacing between your chip and fluidic tubing. See "Read More"for additional information and links.
Are there alternatives to a chip holder?
While alternatives to a chip holder are available, their implementation is complex and not generally recommended. Further information can be found under “Read More”.
Are combi-packs available, or can pack contents be mixed?
We understand your interest in exploring different options; however, it’s important to have a sufficient number of chips of the same type to ensure consistent and reliable experimental results — as one test is not test.
For this reason, we do not offer combi-packs. Mixing the contents of existing packs is also not possible for practical reasons.
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