Amplification of SPPS150 and Salmonella typhi DNA with a high throughput oscillating flow polymerase chain reaction device

Biomicrofluidics, volume 4, Issue 2, 2010

D. Sugumar, Asma Ismail, Manickam Ravichandran, Ismail Aziah, and L. X. Kong. Centre for Material and Fibre Innovation, Deakin University, Waurn Ponds Campus, Geelong, 3217 Victoria, Australia

In this paper, a novel oscillating flow polymerase chain reaction (PCR) device was designed and fabricated to amplify SPPS150 and salmonella typhi. In this new design, the samples are shuttled (oscillating flow) inside a microfluidic chip to three different temperature zones required for DNA amplification. The amplification cycle time has markedly been reduced as the reagent volume used was only about 25% of that used in conventional PCRs. Bubble formation and adsorption issues commonly associated to chip based PCR were also eliminated. Based on the performance evaluated, it is demonstrated that this oscillating flow PCR has the advantages of both the stationary chamber and continuous flow PCR devices.
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