Thin bottom flow cell - topconnect - hydrophobic coating

Application possibilities

A flow cell / observation cell is a sample cell designed so that liquids sample, for instance containing cell’s, proteins or particles, can be continuously flowed though a observation area.
Our glass flow’s cell are intended for situation where the optical properties are extremely critical and are suitable for high resolution imaging. They are often used in combination with fluorescent markers.

As alternative to continuously flowed sample it’s also possible to apply stopped flow observation by adding pressure control on both the cell inlet and outlet.   

Research examples can be for instance:

• In-line sampling of liquids, mostly using a optical read out.
• Cell observation / counting
• Measurement of fluorescent response.  
• Combined with surface chemistry they are used as base for NGS (Next-Generation Sequencing) technology.

Unique selling point.

• The different channel dimensions help to determine the optimal channel size for your experiement.
• The channels doesn’t have any substantial roughness and are fully optical transparent due to the etching process used.
• Glass offers sublime optical transparency and low autofluorescence.
• Manufactured from D263-Bio glass for even lower autofluorescence (fluorescent markers with emissions above 600nm).
• Compatible with #1.5 coverslip

Key features of the products 

Coating
The selected products contains a hydrophobic coating. This coating will change the contact angle between channel surfaces and the liquid.  
This coating can influence the binding of proteins and other biomolecules. There's currently no information available if autofluorescence is influenced. 

Interfacing
This product is compatible with our Fluidic Connect Pro holder for chips supplied in a cartridge.
Because of the limited chip thickness it’s necessary to use the metal strips included with the holder, see here for more information.
To connect all three channels at the same time, you need six ferrules. The ferrules are available in sets of five. Make sure you order two sets if you want to connect three channels at the same time.