Resealable flow cell - topconnect

Cleaning Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket. Glass slides with gasket: Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues. Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost. It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities. Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible. Cleaning with acids or bases has not been fully tested. Glass slides without gasket: Can be cleaned with organic solvents. Can be gently wiped with tissue. Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible. Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water. Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.

Sterilization Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket. Glass slides with gasket: Can be sterilized with ethanol. Dry heat of up to 140 °C can be applied. Can be autoclaved. Glass slides without gasket: Can be sterilized with ethanol. Can be autoclaved.

The oxygen permeability data from the exact material grade used is not available, however the data from a different grade of the same material family, can be found below.

The view window on the bottom of the resealable/OOC chip holder has the dimensions as found in the picture below:  

Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent. Large roughness for structures in glass chips is typical observed for structures manufactured by use of laser assisted manufacturing techniques or abrasion-based techniques like powder blasting. Almost all catalogue products from Micronit are manufactured using wet etching to create full transparent channels without substantial roughness. 

Our OOC chips and resealable flow cells are designed for low-pressure applications, up to 300–400 mbar. The large channels allow for effective flow with minimal pressure, making it crucial to use a pump system that can control and limit pressure within this range. We recommend using a pressure-based system for optimal performance. If a syringe pump is preferred, please ensure that each liquid path includes a pressure relief valve to maintain leak free operating conditions. This setup will allow for cost effective flow control while preventing leakage. An example of such a valve is this.