H-shaped channel for boundary flow experiment - 120 x 50µm - Thin bottom

SKU
11003578
Availability:
check_circle In stock
$463.01

per pack of 3

H-shaped flow channel, ideal for boundary flow experiments.  The channels have a width of 120µm and height of 50µm. A thin bottom layer (#1.5 coverslip) is used to support high resolution imaging. Use of D263-Bio minimizes autofluorescence for most colors of fluorescent dies.

This H-shaped flow channel is an ideal solution for boundary flow experiments in which two liquids will flow in parallel and only diffusion will occur at the interface of both flows. 

The channels are etched into borosilicate glass (D263-Bio which creates extremely smooth channels which are fully transparent.  The glass thickness of the bottom layer is comparable to a #1.5 coverslip. D263-Bio glass offers lowest autofluorescence for most colors of fluorescent markers. Those aspects allows high resolution imaging.  

The channels have a width of 120µm and height of 50µm.
The main channel has a length of 14.8mm, the inlet/outlet structures a length of 14.5mm

The channel profile is as follow:

The chip has a thickness of 1.3mm, the height above the channel is 1050µm.
The height below the channel is 175µm.

More Information
Unit of measurementpack of 3
Interface typeTopconnect
Chip materialBorosilicate glass - D263 bio
Chip thickness1.3mm
Number of inlets2
Number of outlets2
Channel Width120µm
Bottom thickness175µm bottom layer
CoatingNo coating (hydrophilic)
Customer Questions
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements. What can be customized? The channel shape. The channel height and width.For wet etching its important to consider that channel height and minimal channel width are related.<br>{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm}Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels. Surface flatness. We can provide ultra flat surfaces.
When should I use the metal strips (spacers) as provided with the Fluidic Connect Pro holder?
The metal strips (Spacers) are used for thin bottom chips and are also part of the holder for resealable flow cell's. See "Read More" for details.
What's important in selection of a suitable microscope objective?
The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.
What is the roughness of the etched structures?
Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent.
How to clean a chip with coating?
Chips that have Micronit’s standard hydrophobic coating can be cleaned with most organic solvents.
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