Focused flow droplet generator - sideconnect

Name: Focused flow droplet generator - sideconnect
SKU: FF_DROP_SIDE
Price: 329.87 USD

SKU

FF_DROP_SIDE

Availability:

check_circle In stock

As low as $329.87

per pack of 3

Pack of 3 focused flow droplet generator chips for use in combination with our sideconnect interfacing. The chips are available with both a coated (hydrophobic) and uncoated (hydrophilic) channel surface.

Indicated reference droplet sizes are based on a reference experiment with Silicone oil 5cSt and DI water combined with 2% v/v Tween 20 or Tween 80.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Details

Pack of 3 single nozzle side connect droplet generators with a nozzle etched on both sides, giving the following advantages:

Optimized nozzle geometry
Symmetric channels and nozzles for uniform droplet formation
Droplet production stable over a broader size and frequency range
Smaller droplet sizes available

This microfluidic droplet generator is an excellent tool for generating highly reproducible microsized droplets with much higher precision and repeatability compared to conventional methods.

By tuning the relative viscosities, surface tension, and velocities between the dispersed and the continuous phase, droplet size and frequency can be altered. Oil-in-Water (O/W) droplets can be generated directly using the glass chips. Also, these droplet generators are suitable for coating in order to form Water-in-Oil droplets (W/O).

Further specifics:

Droplet size can roughly be tuned from the size of the nozzle to be doubled
Suitable for foam, digital PCR, single cell analysis, emulsions etc.
Made from high quality glass, borosilicate, suitable for most biological and chemical applications

Application examples:

Cell, DNA, bead encapsulation for

drug discovery
drug studies
molecular biological studies
immunology studies
evolutional studies
enzyme catalysis studies

Food, paints, foams

Bubble formation
Mineral Oil Emulsion Production
Particle production - PLGA, PEGDA, gelatine, alginate, polystyrene, agaros
Drug delivery - creams, aerosols

Chemical

Droplet based micromixing
Droplet based microreactions

More Information

More Information
Unit of measurement	pack of 3
Interface type	Sideconnect
Chip material	Borosilicate glass
Chip thickness	1800µm
Channel location	Top and bottom
Number of inlets	2
Number of outlets	1

Documents

Label	Description	Type	Size	Download
DGFF.SC.10 - Drawing	Drawing for 10µm sideconnect droplet generator. Items 01642 and 01365	PDF	297.8 KB	Download
DGFF.SC.75 - Drawing	Drawing for 75µm sideconnect droplet generator. Items 01641and 01368.	PDF	184.2 KB	Download
DGFF.SC.50 - Drawing	Drawing for 50µm sideconnect droplet generator. Items 01640 and 01367.	PDF	198.5 KB	Download
DGFF.SC.05 - Drawing	Drawing for 5µm sideconnect droplet generator. Items 01653 and 01364.	PDF	205.6 KB	Download
Surface wetting properties		pdf	220.3 KB	Download
Flow rate instructions		pdf	303.6 KB	Download
How to prevent clogging		pdf	153 KB	Download
Using the right surfactants		pdf	168.8 KB	Download
Droplet Generator Guide		pdf	617 KB	Download

Product Questions (12)

Customer Questions

How do I clean my uncoated chips?

1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water. 2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages. 3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. 4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.

I need a pumping system for my setup. Which one do you recommend?

We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles.To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers. https://www.fluigent.com/

Which flowrates should I use?

Advice on how to find the right flow rates and to to change the droplet size can be found under "Read More".

I only see streaks of fluids but no droplets. How do I get the droplets?

When you only see streaks of fluids its important to decrease your flowrate. Instructions to find the right flow rate can be found under "Read More".

Which surfactants should I use?

Tween 20, Tween 80 and Emulseo FluoSurf™-O surfactant are used in combination with our chips. See "Read More" for details.

How do I prevent clogging of my chips?

Clogging can only be avoided by working as cleanly as possible. Typical sources of particles include: - Dust from the air or open surfaces - Larger particles or other contamination in the fluids - Dried or solidified residues when re-using chips from previous runs - Components within the fluidic setup (e.g., tubing, connectors, reservoirs) - Residual particles remaining after cleaning See “Read More” for detailed advice on minimizing these risks.

Should I use coated or uncoated droplet generators?

For water-in-oil droplets, use droplet generators with a hydrophobic coating.For oil-in-water droplets, no additional coating is needed. Below more details on surface wetting properties are explained.Surface wetting propertiesThe contact angle between the fluid and the droplet generator surface is key for defining the stability of the wetting of the continuous phase. If the wetting is more preferential for the dispersed phase, pinning of the droplets to the channel can occur. The standard uncoated droplet generators of Micronit are glass based, thus hydrophilic and are suitable for making organic droplets in an aqueous phase (oil-in-water droplets). It is possible for Micronit to provide a coating which renders the surface of the droplet generators hydrophobic, thus suitable for making aqueous droplets in an organic phase (water-in-oil droplets). This coating is based on a fluorinated polymer and ensures the coated surface has a contact angle of more than 90° with water.

I have a Elveflow OB1 pressure based pump what connectors do I need?

You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.

I have a Fluigent pressure based pump, what connectors do I need?

See "Read More" for advice.

What's important in selection of a suitable microscope objective?

The objective working distance is a critical parameter for selection of an objective. See "Read More" for recommendations.

Does those droplet generators work well with Emulseo surfactants and Oils?

Micronit hydrophobic coated Glass Droplet generators are tested with the combination of Emulseo FluoSurf-O™ surfactant and Emulseo FluoOil™ 7500 oil and good results are obtained.

Can I apply a hydrophobic coating myself using Emulseo Fluo-ST3™ surface treatment?

The combination of Micronit glass droplet generators and Emulseo Fluo-ST3™ surface treatment is tested and works well.

We found other products you might like!

Droplet generation setup - sideconnect (no pumps included)

From $1,012.42
To $4,045.14

Focused flow droplet generator - sideconnect

Services

Microfluidic Chips

Expertise

Webstore Resources

About us

Careers