Droplet generation setup - topconnect (no pumps included)

Name: Droplet generation setup - topconnect (no pumps included)
SKU: top_connect_droplet
Price: 2097.9 USD

From $2,097.90

To $12,570.27

Availability: check_circle In stock

$2,097.90

per Bundle

This setup contains a chip holder, a connection kit, a selection of chips and other tools for a quick start.

Holder

1 x Fluidic Connect Pro chip holder - topconnect - for chips in a cartridge + $1,870.76

+ 0

More information

Connection kit

+ 0

Droplet chips [hold Ctrl for multiple]

Optional: Set of two in-line filters (protection of nozzle)

1 x In-line filters + $478.25

+ 0

More information

Optional: Polymeric Tubing Cutter

1 x Polymeric tube cutter + $78.76

+ 0

More information

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Details

This setup does not include pumps. For droplet generation, we recommend pressure based pumps to ensure an optimal experience. Pressure based pumps use stabilized pressure to push liquid out of a reservoir. Syringe pumps can generate a step wise movement in pressure release. This can result in pulsations, that cause instability in droplet generation. There are some high-end syringe pumps on the market that might overcome this issue, but we have limited experience with such pumps. For use in combination with high-end syringe pumps our Syringe to tube connection kit might be a useful addition.

Droplet generation chips are available in the following types:

Focused Flow
T-shaped
Parallel

Focused flow droplet generators are available in the following configurations:

SKU	Nozzle size	Coating	Use for	Recommended droplet size (reference liquids)
00935	10µm	Non - Hydrophilic	Oil-in-water droplets	9µm< droplets <20µm
01341	10µm	Hydrophobic	Water-in-oil droplets	9µm< droplets <20µm
02137 (Beta product)	20µm	Non - Hydrophilic	Oil-in-water droplets	15µm< droplets <30µm
	20µm	Hydrophobic	Water-in-oil droplets	15µm< droplets <30µm
00877	50µm	Non - Hydrophilic	Oil-in-water droplets	40µm< droplets <90µm
01366	50µm	Hydrophobic	Water-in-oil droplets	40µm< droplets <90µm
00580	75µm	Non - Hydrophilic	Oil-in-water droplets	65µm< droplets <140µm
00970	75µm	Hydrophobic	Water-in-oil droplets	65µm< droplets <140µm

T-shaped droplet generators are available in the following configurations:

SKU	Coating	Use for	Channel width	Channel height
FC_TSDG.2_PACK	Non - Hydrophilic	Oil-in-water droplets	100µm	20µm
FC_TSDG.C.2_PACK	Hydrophobic	Water-in-oil droplets	100µm	20µm

Parallel droplet generators combine multiple focused flow droplet generators in parallel on a single chip with a shared inlet and outlet for increased production rates. They are available in the following configurations:

SKU	Nozzle size	Number of junctions	Coating	Use for	Recommended droplet size (reference liquids)
00923	75µm	8 lanes	Non - Hydrophilic	Oil-in-water droplets	65µm<droplets <140µm
02067	75µm	8 lanes	Hydrophobic	Water-in-oil droplets	65µm<droplets <140µm

More Information

More Information
Unit of measurement	Bundle
Application	Droplet generation
Holder concept	Fluidic Connect Pro

Documents

Label	Description	Type	Size	Download
Flow rate instructions		pdf	303.6 KB	Download
Surface wetting properties		pdf	220.3 KB	Download
How to prevent clogging		pdf	153 KB	Download
Using the right surfactants		pdf	168.8 KB	Download
Droplet Generator Guide		pdf	617 KB	Download
TSDG.2 - T-shaped droplet generator		pdf	67.3 KB	Download
DGFF.P8.75 - Parallel droplet generator 75µm, 8 junctions -Drawing		PDF	458.8 KB	Download
DGFF.50 - Drawing	Drawing for 50µm Topconnect Focused Flow Droplet Generator. Items 01366 and 00877.	PDF	179.6 KB	Download
DGFF.75 - Drawing	Drawing for 75µm Topconnect Focused Flow Droplet Generator. Items 00970 and 00580.	PDF	193.6 KB	Download
DGFF.10 - Drawing	Drawing for 10µm Topconnect Focused Flow Droplet Generator. Items 01341 and 00935.	pdf	159.2 KB	Download
Fluidic Connect Pro - Droplet quick start guide	A manual how to assemble the holder for use with a droplet generator.	pdf	5.8 MB	Download

Product Questions (12)

Customer Questions

How do I clean my uncoated chips?

1) Flush an alkaline solution through the channels, like 1 M sodium hydroxide in water. 2) A water bath with ultrasonic agitation can be used to remove particulated matters, keep energy levels initially low to prevent potential damages. 3) Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. 4) Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions.

I need a pumping system for my setup. Which one do you recommend?

We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles.To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers. https://www.fluigent.com/

Which flowrates should I use?

Advice on how to find the right flow rates and to to change the droplet size can be found under "Read More".

I only see streaks of fluids but no droplets. How do I get the droplets?

When you only see streaks of fluids its important to decrease your flowrate. Instructions to find the right flow rate can be found under "Read More".

Which surfactants should I use?

Tween 20, Tween 80 and Emulseo FluoSurf™-O surfactant are used in combination with our chips. See "Read More" for details.

How do I prevent clogging of my chips?

Clogging can only be avoided by working as cleanly as possible. Typical sources of particles include: - Dust from the air or open surfaces - Larger particles or other contamination in the fluids - Dried or solidified residues when re-using chips from previous runs - Components within the fluidic setup (e.g., tubing, connectors, reservoirs) - Residual particles remaining after cleaning See “Read More” for detailed advice on minimizing these risks.

Should I use coated or uncoated droplet generators?

For water-in-oil droplets, use droplet generators with a hydrophobic coating.For oil-in-water droplets, no additional coating is needed. Below more details on surface wetting properties are explained.Surface wetting propertiesThe contact angle between the fluid and the droplet generator surface is key for defining the stability of the wetting of the continuous phase. If the wetting is more preferential for the dispersed phase, pinning of the droplets to the channel can occur. The standard uncoated droplet generators of Micronit are glass based, thus hydrophilic and are suitable for making organic droplets in an aqueous phase (oil-in-water droplets). It is possible for Micronit to provide a coating which renders the surface of the droplet generators hydrophobic, thus suitable for making aqueous droplets in an organic phase (water-in-oil droplets). This coating is based on a fluorinated polymer and ensures the coated surface has a contact angle of more than 90° with water.

Publication: Controlled and tunable polymer particles' production using a single microfluidic device

Amoyav, Benzion, and Ofra Benny. "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10.

Publication: Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids

Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022.

I have a Elveflow OB1 pressure based pump what connectors do I need?

You need a combination of items supplied by Elveflow and Micronit. Please take a look at the full answer for more details.

I have a Fluigent pressure based pump, what connectors do I need?

See "Read More" for advice.

What are the dimensions of the view window in the Fluidic Connect Pro for chips in a cartridge?

The dimension of the view window is roughly 24.4x25.2mm, see "Read More" for details.

Droplet generation setup - topconnect (no pumps included)

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