Chip electrophoresis with mass spectrometric detection in record speed
Lab Chip, 2010, volume 10
Fritzsche S, Hoffmann P, Belder D. Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, 04103, Leipzig, Germany.
We demonstrate that the combination of high speed separations on chip with a fast mass spectrometer enables electrophoretic separations with full mass spectra registration within a second. This was accomplished by coupling a microfluidic glass chip with an integrated nanospray emitter to a fast time of flight mass spectrometer working at 100 Hz for data acquisition. Applying field strengths up to 5800 V cm(-1) we achieved separations of model analytes such as pharmaceuticals and peptides with subsequent acquisition of full mass spectra within one second or slightly more.