Ultrafast DNA sequencing on a microchip by a hybrid separation mechanism that gives 600 bases in 6.5 minutes

PNAS, 2008, January 15, volume 15, no. 2

Christopher P. Fredlake, Daniel G. Hert, Cheuk-Wai Kan, Thomas N. Chiesl, Brian E. Root, Ryan E. Forster, and Annelise E. Barron. Departments of Chemical and Biological Engineering, Chemistry, and Materials Science and Engineering, Northwestern University, Evanston, IL 60208

To realize the immense potential of large-scale genomic sequencing after the completion of the second human genome (Venter’s), the costs for the complete sequencing of additional genomes must be dramatically reduced. Among the technologies being developed to reduce sequencing costs, microchip electrophoresis is the only new technology ready to produce the long reads most suitable for the de novo sequencing and assembly of large and complex genomes. Compared with the current paradigm of capillary electrophoresis, microchip systems promise to reduce sequencing costs dramatically by increasing throughput, reducing reagent consumption, and integrating the many steps of the sequencing pipeline onto a single platform. Although capillary-based systems require

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